E-ISSN 2149-2018 editor@ejmamr.online


Applied Medical Research. 2021; 8(1):(86-107)


Characterization and Immunologic Response of IgY Against Leishmania Mexicana

Marbel Torres-Arias

Abstract
Avian IgYs are polyclonal antibodies resulting from humoral adaptive immune responses. With perspective for many application, IgYs are considered the most versatile biomolecule, able to be used as alternative in biomedicine and as diagnostic tool. Because they are inducible, these antibodies could be produced to potentiate the recognition of relevant clinical pathogens such as Leishmania mexicana. In this investigation anti Leishmania mexicana IgYs were obtained by inoculation of inactivated promastigotes in brown Lohmann hens, at different periods. Twenty-four per cent of the eggs were randomly selected for processing. IgYs were precipitated by Ammonium Sulfate and purified by affinity chromatography. Immunoglobulins with 175 kDa, complete structure, and a concentration of 6.3 mg/mL per yolk were obtained. The most reactive antibodies corresponded to week 8 (W8). These IgYs were tested for specific recognition of their target parasite, using structural and proteins viral as GP63 in Western Blot. Also, they identified concentrations of parasite antigen of 5 and 50 μg/mL with maximal antibody dilution of 1:2000 in ELISA. The IgY-mediated immunodetection offers this as solution to the necessity of anti L. mexicana antibodies for diagnostics and immunotherapy research.
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